Product overview

• Name
  DNQX
• Short description
  Selective, competitive AMPA / kainate receptor antagonist
• Biological description
  Selective and competitive AMPA and kainate receptor antagonist. Also acts as partial AMPA agonist in the presence of γ2 transmembrane AMPA receptor regulatory proteins (TARP) subunit. A neuroleptic agent that displays pro-oxidant activity.
• Biological action
  Antagonist
• Purity
  >98%
• Our products in action
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Properties

• Chemical name
  6,7-Dinitroquinoxaline-2,3-dione
• Molecular Weight
  252.14
• Chemical structure
  
• Molecular Formula
  C₈H₄N₄O₆
• CAS Number
  2379-57-9
• PubChem identifier
  3899541
• SMILES
  C1=C2C(=CC(=C1[N+](=O)[O-])[N+](=O)[O-])NC(=O)C(=O)N2
• Source
  Synthetic
• InChi
  InChI=1S/C8H4N4O6/c13-7-8(14)10-4-2-6(12(17)18)5(11(15)16)1-3(4)9-7/h1-2H,(H,9,13)(H,10,14)
• InChiKey
  RWVIMCIPOAXUDG-UHFFFAOYSA-N
• MDL number
  MFCD00069257
• Appearance
  Pale yellow solid

Applications

• Application notes

  DNQX antagonizes the actions of glutamate at AMPA receptors. It is commonly used to reduce excitatory post synaptic currents (EPSC) and is commonly used at 10 μM. DNQX disodium salt from Hello Bio completely blocks both spontaneous and evoked EPSCs at 10 μM, with concentrations of 1 μM also effective (see Fig 1 above).

   

  #Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of NBQX disodium salt until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of NBQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABA_A-R antagonists.

Storing and Using Your Product

• Storage instructions
  Room temperature
• Solubility overview
  Soluble in DMSO (100mM)
• Important
  This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.