Product overview

• Name
  CNQX disodium salt
• Short description
  Potent, competitive AMPA / kainate receptor antagonist. Disodium salt.
• Biological description

  Potent and competitive AMPA and kainate receptor antagonist. Water soluble, disodium salt. Also antagonizes NMDA receptors at the glycine site. Increases GABA_A receptor spontaneous postsynaptic currents (sPSCs). Shows neuroprotective actions. CNQX also available.

• Biological action
  Antagonist
• Purity
  >98%
• Customer comments

  The CNQX is going fine ! Verified customer, IBPS, Inserm, CNRS

• Our products in action
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Properties

• Chemical name
  6-Cyano-7-nitroquinoxaline-2,3-dione disodium
• Molecular Weight
  276.12
• Chemical structure
  
• Molecular Formula
  C₉H₂N₄O₄Na₂
• CAS Number
  479347-85-8
• PubChem identifier
  2821
• SMILES
  C1=C(C(=CC2=C1N=C(C(=N2)[O-])[O-])[N+](=O)[O-])C#N.[Na+].[Na+]
• Source
  Synthetic
• InChi
  InChI=1S/C9H4N4O4.2Na/c10-3-4-1-5-6(2-7(4)13(16)17)12-9(15)8(14)11-5;;/h1-2H,(H,11,14)(H,12,15);;/q;2*+1/p-2
• InChiKey
  YCXDDPGRZKUGDG-UHFFFAOYSA-L
• MDL number
  MFCD09953908
• Appearance
  Brown or yellow solid

Applications

• Application notes

  The AMPA receptor antagonist CNQX disodium salt is commonly used at concentrations of 10 μM to inhibit the actions of glutamate acting on AMPARs.

  CNQX disodium salt from Hello Bio reduces both spontaneous and evoked EPSCs in cortical neurons at concentrations of 1 μM with full AMPA receptor blockade at 10 μM (see Fig 1 above).

   

  #Protocol 1: Evoked and spontaneous excitatory post synaptic currents (EPSCs)

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  •  EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
  • Neurons were held at -70 to -60 mV (the reversal potential of GABA currents). EPSCs were continuously stimulated and recorded in response to 5 min applications of varying concentrations of CNQX disodium salt until complete receptor inhibition.
  • Spontaneous EPSCs were recorded before and after addition of CNQX disodium salt by holding the neuron at -70 mV and recording for 10 sec.
  • Recordings for EPSCs were made in the absence of GABA_A-R antagonists.

Storing and Using Your Product

• Storage instructions
  Room temperature (desiccate)
• Solubility overview
  Soluble in water (20mM)
• Handling
  Hydroscopic solid, contact with air may cause material to change colour and become sticky. Product performance should not be affected but we recommend storing the material in a sealed jar.
• Important
  This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.