Productoverview

• Name
  CHIR99021trihydrochloride
• Shortdescription
  Potent,selectiveGSK3inhibitor.Wntsignalingactivator.Watersoluble.
• BIOLOGicaldescription

  Potent,selectiveandATP-competitiveGSK3βinhibitor(IC₅₀valuesare6.7and10nMforGSK-3βandGSK-3αrespectively).

  
  Watersolublehydrochloridesaltof CHIR99021.

  
  WntsignalingactivatorwhichiscommonlyusedwithPD032501aspartofthe2icondition.

  
  ExhibitsnocrossreactivityagainstCDKsandexhibits>500-foldselectivityforGSK3overotherproteinkinasesand>800-foldselectivityover20+otherenzymesandreceptors.

  
  Promotesself-renewalofembryonicstemcellsandenablesmouseembryonicfibroblast(MEF)reprogrammingintoiPSCs.

• Biologicalaction
  Inhibitor
• Purity
  >98%
• Ourproductsinaction
  SubmitYourCitationNow

Properties

• Chemicalname
  6-[[2-[[4-(2,4-Dichlorophenyl)-5-(5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl]amino]ethyl]amino]-3-pyridinecarbonitriletrihydrochloride
• MolecularWeight
  574.72
• Chemicalstructure
  
• MolecularFormula
  C₂₂H₁₈Cl₂N₈.3HCl
• CASNumber
  1782235-14-6
• PubChemidentifier
  78243722
• SMILES
  CC1=CN=C(N1)C2=CN=C(N=C2C3=C(C=C(C=C3)Cl)Cl)NCCNC4=NC=C(C=C4)C#N.Cl.Cl.Cl
• Source
  Synthetic
• InChi
  InChI=1S/C22H18Cl2N8.3ClH/c1-13-10-29-21(31-13)17-12-30-22(32-20(17)16-4-3-15(23)8-18(16)24)27-7-6-26-19-5-2-14(9-25)11-28-19;;;/h4-5,8,10-12H,6-7H2,1H3,(H,26,28)(H,29,31)(H,27,30,32);3*1H
• InChiKey
  DSFVSCNMMZRCIA-UHFFFAOYSA-N
• MDLnumber
  MFCD11846251

StoringandUsingYourProduct

• Storageinstructions
  -20°C
• Solubilityoverview
  Solubleinwater(25mM)andinDMSO(50mM)
• Important
  ThisproductisforRESEARCHUSEONLYandisnotintendedfortherapeuticordiagnosticuse.Notforhumanorveterinaryuse.

ReferencesforCHIR99021trihydrochloride

• TherolesofNotch3onthecellproliferationandapoptosisinducedbyCHIR99021inNSCLCcelllines:afunctionallinkbetweenWntandNotchsignalingpathways.

  LiCetal(2013)PLoSOne8(12):e84659.

  PubMedID:24367688

• Selectiveglycogensynthasekinase3inhibitorspotentiateinsulinactivationofglucosetransportandutilizationinvitroandinvivo.

  RingDBetal(2003)Diabetes52(3):588-95.

  PubMedID:12606497

• Pleiotropyofglycogensynthasekinase-3inhibitionbyCHIR99021promotesself-renewalofembryonicstemcellsfromrefractorymousestrains.

  YeSetal(2012)PLoSOne7(4):e35892.

  PubMedID:22540008