你好Bio/CA200992 CellAura荧光5-HT<sub>1A</sub>拮抗剂[NAN-190]/HB7828/-免疫在线蚂蚁淘旗下平台

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商品详细你好Bio/CA200992 CellAura荧光5-HT_1A拮抗剂[NAN-190]/HB7828/

你好Bio/CA200992 CellAura荧光5-HT_1A拮抗剂[NAN-190]/HB7828/

商品编号： HB7828

品牌： hellobio

市场价： ¥2086.50

美元价： 1605.00

产地：美国(厂家直采)

公司：

产品分类：酸碱缓冲液

公司分类： acid_base_buffer_solution

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Productoverview

Name
CA200992CellAurafluorescent5-HT_1AantagoNIST[NAN-190]
Shortdescription
Fluorescent5-HT_1Areceptorantagonist
BIOLOGicaldescription
Fluorescent5-HT_1ASEROtoninreceptorantagonist(apparentK_Dvaluesare8.75,6.34and5.57for5-HT_1A,5-HT_2Aand5-HT_1Breceptorsrespectively).Antagonizestheactivityofserotonin,a5-HT_1Aagonist.
Alternativenames
CA200992|5HT1A-633-AN2
Biologicalaction
Antagonist
Purity
>97%
Ourproductsinaction
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Properties

MolecularWeight
1025
Source
Synthetic
Formulation
Lyophilizedfilm
Excitation
633nm
Emission
650nm

Applications

Applicationnotes
Forimagingatthe5-HT_1Areceptorusesolutionsupto200nM.
Pharmacologicalvalidation
TheCellAurafluorescent5-HT_1Aantagonist[NAN-190]ligandwasshowntoantagonizetheactivityofthe5‑HT1Aagonist,5-HT,intheCHOcelllineexpressingthehuman5-HT_1Areceptor,usingtheCa²⁺sensitivefluorescentindicator,Fura-2AM.Cellswereplatedinclearbottomblack96-welltissuecultureplatesandgrowntoconfluencefor24hours.MediumwasthenremovedandcellsincubatedinloADIngbuffercomprisingHEPESbufferedsalinewith0.5mMBrilliantBlackBN,2.5mMprobenecid,0.023%pluronicacidF-127and2.5µg/mlFura-2AMat37ºCfor30–45mins,eitherinthepresenceofCellAurafluorescent5-HT_1Aantagonist[NAN-190]oraDMSOvehiclecontrol.Duringthistimeserialdilutionsofthe5‑HT1Aagonist,5-HT,werepreparedinHEPESbufferedsalinecontaining2.5mMprobenecidand0.5mMBrilliantBlackBN.TodeterminetheantagonistactivityofCellAurafluorescent5-HT_1Aantagonist[NAN-190],serialdilutionsoftheagonistwasaddedtowellswithorwithoutCellAurafluorescent5-HT_1Aantagonist[NAN-190].TheagonistCa²⁺responseintheabsenceorpresenceofCellAurafluorescent5-HT_1Aantagonist[NAN-190]wasdeterminedonaMolecularDevicesFlexStationbyexcitingat340nmand380nmandratioingthefluorescenceintensityofFura-2signalcollectedat320nm.TheapparentKDwascalculatedfromtherightwardshiftoftheagonistresponsecurveinthepresenceofCellAurafluorescent5-HT_1Aantagonist[NAN-190],comparedtotheresponsecurvefortheagonistalone.

StoringandUsingYourProduct

Storageinstructions
-20°C,protectfromlight
Solubilityoverview
SolubleinDMSO
Handling
Afterthawingindividualaliquotsforuse,werecommendbrieflysonicatingthesampletoensureitisfullydissolvedandthesolutionishomogeneous.Wedonotrecommendusingtheproductaftersubjectingittorepetitivefreeze-thawcycles.
Shippingconditions
Theproduct,suppliedinadryform,isstableatambienttemperatureforperiodsofuptoafewdaysanddoesnotrequireshippingonice/dryice.
Important
ThisproductisforRESEARCHUSEONLYandisnotintendedfortherapeuticordiagnosticuse.Notforhumanorveterinaryuse.

品牌介绍

Hello Bio是由一群经验丰富的科学家和化学家们创立的充满活力的生物企业，总部位于英国布里斯托尔，在美国普林斯顿设有分公司，主旨在于提供质优价廉的生命科学研究工具，主要产品线为小分子化合物，如激动剂、拮抗剂、抑制剂等，部分产品价格甚至低至其他供应商的50%。热销产品线：酶抑制剂和激动剂ROCK inhibitor Y-27632, PKA inhibitor KT 5720 , adenylyl cyclase activator Forskolin, and novel GRK2/3 inhibitor Cmpd101等。离子通道调节剂NMDA antagonist D-AP5, sodium channel blocker tetrodotoxin citrate, TRP antagonist capsazepine, and GABAA antagonist SR 95531 等。信号通路工具各种信号通路抑制剂、激动剂表观遗传学研究工具potent HDAC inhibitor Trichostatin A, DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine,BET bromodomain inhibitor (+)-JQ1 and potent,selective GLP / G9a HMTase inhibitor BIX 01294 等受体配体novel selective GRK2/3 inhibitor, NMDA antagonist D-AP5, Group I mGlu agonist (S)-3,5-DHPG, and GABAA antagonist SR 95531.等干细胞调节剂ROCK inhibitor Y-27632, SB 431542 DAPT.

公司介绍

品牌分类

拮抗剂和抑制剂抗生素激动剂和活化剂初级的染料和污渍

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