Product overview

• Name
  D-AP5
• Short description
  Selective, competitive NMDA receptor antagonist
• Biological description

  Widely used, selective and competitive NMDA receptor antagonist which binds at glutamate site. More active form of DL-AP5. Blocks induction of LTP (long term potentiation) and impairs spatial learning.

• Alternative names
   2-APV, D-APV
• Biological action
  Antagonist
• Purity
  >99%
• Customer comments

  I made the discovery that the NMDA receptor is the trigger for the induction of LTP using D-AP5 synthesized by Jeff Watkins, the discoverer of the NMDA receptor... I now obtain my D-AP5 from Hello Bio. I love their products and ethos and that is why I accepted a position on their Scientific Advisory Board.

  Professor Graham Collingridge, winner of The Brain Prize, 2016

  My lab used D-AP5 from Hello Bio and were very happy with it. It behaved exactly as expected! Professor Kei Cho, Chair of Neuroscience, University of Bristol, UK (Hello Bio Scientific Advisory Board Member)

  My lab is very satisfied with your D-AP5 quality and price. Verified customer, European Brain Research Institute (EBRI)

• Our products in action
  7 citations Submit Your Citation Now

Images

Properties

• Chemical name
  D-(-)-2-Amino-5-phosphonopentanoic acid
• Molecular Weight
  197.13
• Chemical structure
  
• Molecular Formula
  C₅H₁₂NO₅P
• CAS Number
  79055-68-8
• PubChem identifier
  135342
• SMILES
  N[C@H](CCCP(=O)(O)O)C(=O)O
• Source
  Synthetic
• InChi
  InChI=1S/C5H12NO5P/c6-4(5(7)8)2-1-3-12(9,10)11/h4H,1-3,6H2,(H,7,8)(H2,9,10,11)/t4-/m1/s1
• InChiKey
  VOROEQBFPPIACJ-SCSAIBSYSA-N
• MDL number
  MFCD00078839
• Appearance
  White solid

Applications

• Application notes

  The NMDA receptor antagonist D-AP5 is commonly used to inhibit NMDA mediated synaptic plasticity. It is often used at concentrations of 50 μM. D-AP5 from Hello Bio completely abolishes evoked NMDAR mediated currents at 50 μM and reduces NMDA currents at lower concentrations of 1 and 10 μM (see Fig 1 above).

   

  #Protocol 1: Evoked NMDA receptor currents

  • Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
  •  NMDA currents were evoked via a stimulating electrode placed in layers II/III and evoked by a single square (150 μs) pulse every 10 sec at a stimulus intensity that gave a reliable NMDA current.
  • Neurons were held a +40 mV to relieve NMDA currents from their voltage-dependent Mg²⁺ block.
  • NMDA currents were continually stimulated and recorded in response to continual bath applications of D-AP5 until NMDA currents were completely abolished.
  • All NMDAR recordings were made in the presence of GABA_A-R and AMPAR antagonists.

   

Storing and Using Your Product

• Storage instructions
  Room temperature
• Solubility overview
  Soluble in water (100mM)
• Important
  This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.