Hello Bio/NMDA/HB0454/
市场价：
¥721.50
美元价：
555.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product overview
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NameNMDA
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Short descriptionPrototypic NMDA receptor agonist
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Biological descriptionPrototypic NMDA receptor agonist which mimics the action of glutamate. Potent excitant.
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Biological actionAgonist
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Purity>99%
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Images
Properties
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Chemical nameN-Methyl-D-aspartic acid
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Molecular Weight147.13
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Chemical structure
![NMDA [6384-92-5]](images/hellobio/201710/hb0454.png "NMDA [6384-92-5]")
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Molecular FormulaC5H9NO4
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CAS Number6384-92-5
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PubChem identifier22880
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SMILESCN[C@H](CC(=O)O)C(=O)O
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InChiInChI=1S/C5H9NO4/c1-6-3(5(9)10)2-4(7)8/h3,6H,2H2,1H3,(H,7,8)(H,9,10)/t3-/m1/s1
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InChiKeyHOKKHZGPKSLGJE-GSVOUGTGSA-N
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MDL numberMFCD00004226
Applications
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Application notes
The prototypic NMDA receptor agonist NMDA is effective at a range of concentrations and typically used at 100 μM. NMDA from Hello Bio induces inward depolarising whole-cell currents in cortical neurons at 10 μM with prominent currents at 50 μM. The actions of NDMA were fully blocked by D-AP5 (NMDAR antagonist) at 100 μM (see Fig 1 above).
#Protocol 1: NMDA mediated whole-cell currents
- Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
- Neurons were held at -70 mV and continuously perfused with aCSF in the presence of AMPA and GABA receptor antagonists CNQX (10 μM) and Bicuculline (100 μM) respectively and Tetrodotoxin (1 μM) to reduce network activity.
- NMDA currents were evoked by applying NMDA directly to the recording chamber during continuous perfusion.
• To test the selectivity of NMDA to NMDA receptors the experiment was repeated within the same neuron in the presence of the NMDA receptor antagonist D-AP5 (100 μM). Under these conditions NMDA failed to induce a depolarising current.
Storing and Using Your Product
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Storage instructionsRoom temperature
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Solubility overviewSoluble in water (100mM)
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ImportantThis product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for NMDA
N-methyl-D-aspartic acid (NMDA) in the nervous system of the amphioxus Branchiostoma lanceolatum.
D'Aniello S et al (2007) BMC Neurosci 8 : 109PubMedID: 18096065Regulation of N-methyl-D-aspartic acid (NMDA) receptors by metabotropic glutamate receptor 7.
Gu Z et al (2012) J Biol Chem 287(13) : 10265-75.PubMedID: 22287544Occurrence of D-aspartic acid and N-methyl-D-aspartic acid in rat neuroendocrine tissues and their role in the modulation of luteinizing hormone and growth hormone release.
D'Aniello A et al (2000) FASEB J 14(5) : 699-714.PubMedID: 10744627
品牌介绍
Hello Bio是由一群经验丰富的科学家和化学家们创立的充满活力的生物企业，总部位于英国布里斯托尔，在美国普林斯顿设有分公司，主旨在于提供质优价廉的生命科学研究工具，主要产品线为小分子化合物，如激动剂、拮抗剂、抑制剂等，部分产品价格甚至低至其他供应商的50%。热销产品线：酶抑制剂和激动剂ROCK inhibitor Y-27632, PKA inhibitor KT 5720 , adenylyl cyclase activator Forskolin, and novel GRK2/3 inhibitor Cmpd101等。 离子通道调节剂NMDA antagonist D-AP5, sodium channel blocker tetrodotoxin citrate, TRP antagonist capsazepine, and GABAA antagonist SR 95531 等。信号通路工具各种信号通路抑制剂、激动剂表观遗传学研究工具potent HDAC inhibitor Trichostatin A, DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine,BET bromodomain inhibitor (+)-JQ1 and potent,selective GLP / G9a HMTase inhibitor BIX 01294 等受体配体novel selective GRK2/3 inhibitor, NMDA antagonist D-AP5, Group I mGlu agonist (S)-3,5-DHPG, and GABAA antagonist SR 95531.等干细胞调节剂ROCK inhibitor Y-27632, SB 431542 DAPT.
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