你好生物/环噻嗪/HB0221/50mg
商品编号：
HB0221-50mg
品牌：
hellobio
市场价：
¥2437.50
美元价：
1875.00
产品分类：
酸碱缓冲液
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Product overview
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NameCyclothiazide
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Short descriptionAMPA receptor positive allosteric modulator. Inhibits AMPAR desensitization.
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Biological descriptionAMPA receptor positive allosteric modulator (PAM). Produces a fast inhibition of AMPAR desensitization and a slow potentiation of the AMPA current. Also inhibits GABAA mediated currents. Shows diuretic and convulsive actions. Active in vivo.
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Alternative namesCTZ
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Biological actionPAM
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Purity>98%
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Our products in action2 citations Submit Your Citation Now
Images
Properties
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Chemical name6-Chloro-3,4-dihydro-3-(5-norbornen-2-yl)-2H-1,2,4-benzothiazidiazine-7-sulfonamide-1 ,1-dioxide
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Molecular Weight389.87
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Chemical structure
![Cyclothiazide [2259-96-3]](images/hellobio/201710/hb0221.png "Cyclothiazide [2259-96-3]")
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Molecular FormulaC14H16ClN3O4S2
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CAS Number2259-96-3
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PubChem identifier2910
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SMILESC1C2CC(C1C=C2)C3NC4=CC(=C(C=C4S(=O)(=O)N3)S(=O)(=O)N)Cl
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SourceSynthetic
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InChiInChI=1S/C14H16ClN3O4S2/c15-10-5-11-13(6-12(10)23(16,19)20)24(21,22)18-14(17-11)9-4-7-1-2-8(9)3-7/h1-2,5-9,14,17-18H,3-4H2,(H2,16,19,20)
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InChiKeyBOCUKUHCLICSIY-UHFFFAOYSA-N
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MDL numberMFCD00210192
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AppearanceWhite solid
Applications
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Application notes
The AMPAR positive allosteric modulator Cyclothiazide increases the open time of the AMPAR by inhibiting AMPAR desensitization. It is often used at a concentration of 100 μM. Cyclothiazide from Hello Bio potentiates AMPAR mediated evoked EPSCs in cortical neurons at 50 μM and above (see Fig 1 above)
#Protocol 1: Evoked and spontaneous evoked excitatory post synaptic currents (EPSCs)
- Whole cell voltage clamp recordings were obtained from layer V neurons of the mouse prelimbic cortex brain slice.
- EPSCs were evoked via a stimulating electrode placed in layers II/III delivering a single square (150 μs) pulse every 10 sec at an intensity that gave a reliable EPSC.
- Neurons were held at -70 mV (the reversal potential of GABA currents).
- EPSCs were then continuously stimulated and recorded in response to 5 min applications of 50 μM and then 100 μM Cyclothiazide.
Storing and Using Your Product
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Storage instructionsRoom temperature
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Solubility overviewSoluble in DMSO (100mM) or ethanol (25mM)
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ImportantThis product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for Cyclothiazide
Effects of cyclothiazide on GluR1/AMPA receptors.
Fucile S et al (2006) Proc Natl Acad Sci U S A 103(8) : 2943-7.PubMedID: 16473938Downregulated GABA and BDNF-TrkB pathway in chronic cyclothiazide seizure model.
Kong S et al (2014) Neural Plast 2014 : 310146PubMedID: 24757570The norbornenyl moiety of cyclothiazide determines the preference for flip-flop variants of AMPA receptor subunits.
Kessler M et al (2000) Neurosci Lett 287(2) : 161-5.PubMedID: 10854736Superactivation of AMPA receptors by auxiliary proteins.
Carbone and Plested (2016) Nat Commun 7 : 10178. doi: 10.1038/ncomms1017PubMedID: 26744192
品牌介绍
Hello Bio是由一群经验丰富的科学家和化学家们创立的充满活力的生物企业，总部位于英国布里斯托尔，在美国普林斯顿设有分公司，主旨在于提供质优价廉的生命科学研究工具，主要产品线为小分子化合物，如激动剂、拮抗剂、抑制剂等，部分产品价格甚至低至其他供应商的50%。热销产品线：酶抑制剂和激动剂ROCK inhibitor Y-27632, PKA inhibitor KT 5720 , adenylyl cyclase activator Forskolin, and novel GRK2/3 inhibitor Cmpd101等。 离子通道调节剂NMDA antagonist D-AP5, sodium channel blocker tetrodotoxin citrate, TRP antagonist capsazepine, and GABAA antagonist SR 95531 等。信号通路工具各种信号通路抑制剂、激动剂表观遗传学研究工具potent HDAC inhibitor Trichostatin A, DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine,BET bromodomain inhibitor (+)-JQ1 and potent,selective GLP / G9a HMTase inhibitor BIX 01294 等受体配体novel selective GRK2/3 inhibitor, NMDA antagonist D-AP5, Group I mGlu agonist (S)-3,5-DHPG, and GABAA antagonist SR 95531.等干细胞调节剂ROCK inhibitor Y-27632, SB 431542 DAPT.
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