Product overview

• Name
  (R,S)-AMPA
• Short description
  Prototypic AMPA receptor agonist
• Biological description

  Prototypic AMPA receptor agonist (EC₅₀ = 11 μM). (S)-AMPA is the active enantiomer form.

• Biological action
  Agonist
• Purity
  >98%
• Our products in action
  Submit Your Citation Now

Images

Properties

• Chemical name
  (RS)-α-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid
• Molecular Weight
  186.17
• Chemical structure
  
• Molecular Formula
  C₇H₁₀N₂O₄
• CAS Number
  74341-63-2
• PubChem identifier
  1221
• SMILES
  CC1=C(C(=O)NO1)CC(C(=O)O)N
• Source
  Synthetic
• InChi
  InChI=1S/C7H10N2O4/c1-3-4(6(10)9-13-3)2-5(8)7(11)12/h5H,2,8H2,1H3,(H,9,10)(H,11,12)
• InChiKey
  UUDAMDVQRQNNHZ-UHFFFAOYSA-N
• MDL number
  MFCD00213388
• Appearance
  White solid

Applications

• Application notes

  The AMPA receptor agonist (R,S)-AMPA is typically used at concentrations of 1-100 µM. At 10 µM, (R,S)-AMPA from Hello Bio induces a large depolarising current. This depolarising current was occluded in the presence of the AMPA receptor antagonist NBQX (20µM). (See Fig 1 above).

  
  #Protocol 1: (R,S)-AMPA protocol

  • Whole cell voltage clamp recordings of CA1 pyramidal neurons from the rat hippocampal brain slice.
  • Neurons were held at -60 mV and continuously perfused with aCSF in the presence of the GABA receptor antagonist gabazine (20µM).
  • AMPA currents were evoked via applying (R,S)-AMPA directly to the recording chamber during continuous perfusion.
  • To test the selectivity of (R,S)-AMPA to AMPA receptors, the experiment was repeated within the same neuron in the presence of the AMPA receptor antagonist NBQX (20 µM)
  • Under these conditions (R,S)-AMPA failed to induce a depolarising current.

Storing and Using Your Product

• Storage instructions
  Room temperature
• Solubility overview
  Soluble in water (10mM, gentle warming)
• Important
  This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.